The original transformation protocol dates back to 1970 (Mandel and Higa,J. Mol Biol 1970 ,53; 159). Just preparing the cells took two days.With a protocol two hours long, ontaining multiple steps,it was a revolution in molecular biology.Does this sound familiar?
That’s because it’s still the most popular protocol in use today.
RBC HIT Competent Cells 1 Min competent cells are still currently the fastest transforming cells worldwide since it was launched in 2006.
Due to our unique production process cells can be transformed to high efficiency in 1-10 minutes using a simple 1 step patented protocol.RBC provides a wide range of popular
strains for all your cloning and expression needs.Transform your work with RBC HIT Competent Cells.
|F- ( 80d lacZ M15)|
hsdR17(r – m +)
|F’ traD36 proA+|
recA1 endA1 relA1
|hsdR17(rk- mk+), recA1,|
endA1, gyrA96, thi-1,
supE44, relA1, lac[F’
|end A||Prevents plasmid degradation during extraction||Yes||Yes||Yes||No|
|recA||Prevents DNA recombination||Yes||Yes||Yes||No|
|hsd||Enhances transformation e ciency of selected PCR DNA strands and cDNA libraries||Yes||Yes||Yes||Yes|
|deoR||Enhances transformation e ciency of high MW plasmids and cosmids||Yes||No||No||No|
|LacZ M15||Blue-White screening||Yes||Yes||Yes||No|
|Lon||Lon Protease De cient, Improves Protein Yield||No||No||No||Yes|
|ompT||OmpT Protease De cient, Improves Protein Yield||No||No||No||Yes|