DESCRIPTION:
The 96-well Plasmid Kit is designed for rapid high throughput isolation of plasmid or cosmid DNA
from 1- 2 ml of bacterial cultures. All components and reagents are provided for a complete
solution. In the process, the modified alkaline lysis method and RNase A treatment are used to
obtain cleared cell lysate with minimal genomic DNA and RNA contaminants. In the presence of
a chaotropic salt, the plasmid DNA in the lysate binds to glass fiber matrix in the Plasmid Binding
Plate. The contaminants are washed off with an ethanol based wash buffer and finally, the purified
plasmid DNA is eluted by low salt elution buffer or water. The protocol does not require DNA phenol
extraction or alcohol precipitation. Typical yields are 5-10 μg for high-copy number plasmid and
0.5-5μg for low-copy number plasmids.
