DESCRIPTION:
BCA Protein Assay Kit is developed based on one of the most widely used method for protein concentration assay-Bicinchoninic Acid (BCA) method.
It provides a convenient format for stable and sensitive detection and quantitation of total protein.
This method combines the well-known reduction of Cu +2 to Cu +1 by protein in an alkaline medium
(the biuret reaction)
with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu +1 )
using a unique reagent containing bicinchoninic acid.
The purple-colored reaction product of this assay is formed by the chelation of two molecules of BCA with one cuprous ion. For research use only
This water-soluble complex exhibits a strong absorbance at 562 nm that is nearly linear with increasing protein concentrations.
This kit contains ready-to-use coomassie G-250 dye and bovine serum albumin as protein standards.
The working range is 20-2,000 μg/ml.
