The One-tube RT-PCR Hot Start kit are designed especially for simple and effective . The reverse
transcription step is directed by M-MLV RT (H-). The PCR reaction is by a chemically modified
“Hot-Start” Taq , is activated by heating up the reaction 10 min at 94°C, the Hot start Taq enzyme
is activated. An especially reaction buffer provides both for Reverse Transcriptase and for Hot
Start Taq DNA Polymerase.