DESCRIPTION:
Total RNA Spin Kit (Blood & Cultured Cells) is special designed for purification of total RNA from
cultured cells and fresh human whole blood. And Total RNA Spin Kit (Tissue) is special designed
for purification of total RNA from a variety of animal tissues or cells. Provided micropestle can
efficiently homogenize tissue sample in microcentrifuge tube. The method use detergents and a
chaotropic salt to lyse cell and inactivate RNase, than RNA in chaotropic salt is bonded to glass
fiber matrix of column. After washing off the contaminants, the purified RNA is eluted by RNasefree
water. The entire procedure can be completed in 20 -40 minutes ,ssRNA and dsRNA of
> 200 bps to 1000’s of bps in length are efficiently purified.and the purified RNA is ready for
RT-PCR, northern blotting,cDNA library construction and primer extension.
