The 50 bp DNA ladder consists of 17 liner double stranded DNA bands
of 50, 100, 150, 200, 250, 300, 350, 400, 450, 500, 600,700,800,900,
1000,1200 and 1500 base pair. This 50bp DNA ladder is specially
prepared for visualizing of the DNA fragments on the gel. The intensity
of 200 bp band ,500bp and 1200bp has been increased to yield reference
PCR products and double-stranded DNA digested with appropriate
restriction enzymes, are phenol extracted and equilibrated to 10mM
Tris-HCl (pH 8.0) and 10mM EDTA.