BIOLOGICAL ACTIVITY:
Hygromycin B is an aminoglycoside antibiotic active against prokaryotic and eukaryotic cells. IC50 & Target: Target：Antibacterial;
Antifungal[1] In Vitro: Hygromycin B, an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, is synthesized by
Streptomyces hygroscopicus[1]. Hygromycin B at 0.38 mM concentration completely halts yeast cell growth in rich media, presumably
by preventing protein synthesis by cytoplasmic ribosomes. Polypeptide synthesis in cell-free extracts from rabbit reticulocytes, wheat
germ and yeast is strongly blocked by low concentrations ofhygromycin B. The antibiotic inhibits peptide chain elongation by yeast
polysomes by preventing elongation factor EF-2-dependent translocation. The inhibition of translocation by hygromycin B might result
from the stabilization of peptidyl-tRNA bound to the ribosomal acceptor site[2]
. In Vivo: Hygromycin B inhibits protein synthesis by
blocking ribosomal translocation without causing significant misreading in vivo[3]. Constitutive expression of the bacterial hygR gene in
transgenic mice in vivo confers resistance to hygromycin B[4]
.
PROTOCOL (Extracted from published papers and Only for reference)
Animal Administration: [4]Hygromycin B is dissolved in sterile water. The mice C57BL/6J-TgN(pPWL512hyg)1Ems carrying hygR
are treated with a single dose of hygromycin B i.p. at doses that starts at 2.7 mg/kg and increases by 50% for each consecutive dose.
Control wild-type C57BL/6J mice are treated with the same volume of sterile saline. Total volume injected is 0.5 mL. The health status
and body weights of animals are monitored daily for 10 consecutive days[4]
.
References:
[1]. Pardo JM, et al. Biochemical basis of resistance to hygromycin B in Streptomyces hygroscopicus–the producing organism. J Gen Microbiol. 1985
Jun;131(6):1289-98.
[2]. González A, et al. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochim Biophys Acta. 1978 Dec
21;521(2):459-69.
[3]. Pfister P, et al. Role of 16S rRNA Helix 44 in Ribosomal Resistance to Hygromycin B. Antimicrob Agents Chemother. 2003 May;47(5):1496-502
