DESCRIPTION:
Pfu DNA polymerase is a thermostable enzyme isolated from Pyrococcus furiousus. The enzyme
replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5′-3′
direction. Pfu DNA polymerase possesses 3′-5′ exonuclease (proofreading) activity. Base
misinsertions that may occur during polymerization are rapidly excised by the proofreading activity
of the polymerase. Pfu DNA polymerase is recommended for use in PCR and primer extension
reactions that require high-fidelity synthesis. Pfu DNA polymerase-generated PCR fragments are
blunt-ended.
