PowerAmp 2X PCRmix-Green

Description :

Hot start Taq DNA Polymerase for qPCR is designed for Real-Time PCR and Hot-start PCR. A
special inhibition the reaction at room temperature until after the first denaturation step. This prevents primerdimers
and other artefacts.
PowerAmp 2X PCRmix-Green is optimized mixture contain of Hot Start Taq enzyme, reaction buffer, dNTP,
enhancer as 2-fold concentration. PowerAmp 2X PCRmix-Green is designed to allow the user for quick ,
easy preparation of reaction mixture.The PowerAmp 2X PCRmix-Green can be amplification PCR products
up to 5 kb and the products can be directly cloning into T-vector.

Primers: Use 0.3 μM per primer as a general starting point. For larger amounts of template (e.g., 200 ng genomic
DNA), increasing the concentration up to 0.5 μM per primer may improve yield.
Annealing Temperature: The annealing temperature is slightly higher than with typical PCR. The optimal annealing
temperature should be ~2°C lower than the Tm of the primers used. A range of 58–68°C is recommended.
Extension Time: As little as 30 seconds per kb is suitable for most targets. Use up to 60 seconds per kb for
maximum yield.

PCR Protocol:
1.Thaw the 2x Hot start mix at room temperature. Vortex
the 2x Hot start mix and then spin it briefly in a micro
centrifuge to collect the material in the bottom of the tube.
2. Prepare one of the following reaction mixes on ice:

3. If necessary you can scale up your volume

Program the thermal cycler as follows

Step
After cycling, maintain the reaction at 4°C. Samples can
be stored at –20°C until use.
Analyze products using standard agarose gel electrophoresis.