A complete lysis buffer for the release of cytoplasmic, membrane and nuclear proteins from adherent
and suspension cultured mammalian cells. The RIPA lysis buffer is fully compatible with many
applications, including reporter assays, protein assays, immunoassays and other protein purification
techniques. RIPA Lysis Buffer does not contain protease inhibitors,
however it is fully compatible with our range of individual protease
inhibitors and cocktails.
1X formulation and consisting of 50 mM Tris-HCl, pH 7.5 ,
150 mM NaCl, 1% Nonidet P40,0,5% sodium deoxycholat,
Use 1 ml of cold RIPA Buffer for every 5 x106 of HeLa or
A431 cells (~20 ul of packed cells, which is equivalent to
~40 mg of cells). To obtain concentrated protein extracts,
directly lyse cells on plate and use less buffer.