A recombinant BioTaq DNA polymerase. is a thermostable enzyme of approximately 95kD a expressed
from E-coli .This enzyme replicates DNA at 74 oCand exhibits a half-life of 40 mins
at 95 oC.The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5′–3′
direction in the presence of magnesium and also possesses a 5′-3’exonuclease activity. The PCR
product can be as large as 3-5kb.