Glutathione SepFast is an affinity chromatography medium used for the rapid one-step purification of Glutathione S-transferase (GST)-tagged proteins. Other Glutathione S-transferases and Glutathione-binding proteins can also be purified with this product.
Glutathione SepFast is an affinity chromatography medium used for the rapid one-step purification of Glutathione S-transferase (GST)-tagged proteins. Other Glutathione S-transferases and Glutathione-binding proteins can also be purified with this product.
Glutathione SepFast MAG is a magnetic version that can be processed in aid of a magnet.
Reduced Glutathione ligand is immobilised to the carefully designed porous support via very stable thiolether linkage.
GST-tagged proteins expressed in bacteria, yeasts, insects and mammalian cell cultures can be readily purified in a single purification step. GST tag can be cleaved in bound condition or in eluted condition by specific proteases.
Glutathione SepFast products are specifically designed and fabricated for the purification of GST-tagged proteins in batch (stirred tank), gravity flow or packed column modes. Glutathione SepFast is made of highly cross-linked agarose beads. Its carefully controlled pore structure allows fast access of affinity ligands by target protein molecules. Also, its high mechanical strength permits liquid to pass through gravity columns or packed columns at excellent flow rates. Clarified or unclarified cell lysates (for intracellular proteins) or culture broths (for extracellular proteins) could be directly processed with Glutathione SepFast or Glutathione SepFast MAG. All these translate into high process flexibility and higher protein yield at shortened purification time.
Glutathione SepFast is supplied as loose resin or is supplied as pre-packed columns.
FEATURES
- Affinity Chromatography Medium: For the rapid one-step purification of GST-tagged proteins.
- Ligand: Reduced Glutathione immobilized via stable thiolether linkage to a porous support.
- Resin Composition: High cross-linked agarose beads with controlled pore structure.
- Magnetic Option: Available as Glutathione SepFast MAG, with magnetic beads for processing with a magnet.
- High Binding Capacity: Efficient binding and elution of GST-tagged proteins.
- Flexible Process Modes: Suitable for batch (stirred tank), gravity flow, and packed column chromatography.
- pH Stability: Stable pH range of 3-13 (short term 2-14).
- Resin Regenerability: Resin can be regenerated for multiple uses.
- High Mechanical Strength: Enables excellent flow rates and durability in packed columns or gravity flow setups.
| Product Name | Specifications | Packaging | Additional Notes |
|---|---|---|---|
| Glutathione SepFast | High cross-linked agarose beads | Loose resin | For GST-tagged protein purification |
| Glutathione SepFast MAG | Magnetic version of Glutathione SepFast | Loose resin | Magnetic processing option |
| Glutathione SepFast Columns | Pre-packed columns with resin | Pre-packed columns | Ready-to-use for GST-tagged protein purification |
