T4 DNA Ligase

Storage: -20℃

10X Ligation Buffer:
Contain with Tris-HCl, MgCl2, DTT, ATP and PEG (pH 7.8 at 25 oC)

Inactivation: By heating at 65oC for 10min.

Applications:
Joining double-stranded DNA with cohesive or blunt termini and 3’T-A overhangs.