Description:
Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of three phosphatase inhibitors for the inhibition of both serine/threonine and alkaline phosphatases. Each vial contains a 1 ml solution with the following components: 500 µM Cantharidin, 2.5 mM (-)-p-Bromotetramisole oxalate, and 1 µM Calyculin A form Discodermia calyx.
Phosphatase Inhibitor Cocktail III has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in DMSO.
Storage:
The product should be stored at -20°C, and is stable for up to 12 months. When kept at 2-8°C, the product remains stable for 2 months.
Protocol:
Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.
Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
